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Effect of pollution on DNA damage and essential fatty acid profile in Cirrhinus mrigala from River Chenab

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Chinese Journal of Oceanology and Limnology Aims and scope Submit manuscript

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Abstract

The objective of this study was to evaluate the effect of anthropogenic pollution on DNA damage and the fatty acid profile of the bottom dweller fish (Cirrhinus mrigala), collected from the River Chenab, in order to assess the effect of the toxicants on the quality of the fish meat. The levels of Cd, Hg, Cu, Mn, Zn, Pb, Cr and Sn and of phenols from this river were significantly higher than the permissible limits set by the USEPA. Comet assays showed DNA damage in Cirrhinus mrigala collected from three different sampling sites in the polluted area of the river. Significant differences were observed for DNA damage through comet assay in fish collected from polluted compared to control sites. No significant differences were observed for DNA damage between farmed and fish collected from upstream. The micronucleus assay showed similar trends. Fish from the highly polluted sites showed less number of fatty acids and more saturated fatty acids in their meat compared to fish from less polluted areas. Several fatty acids were missing in fish with higher levels of DNA in comet tail and micronucleus induction. Long-chain polyunsaturated fatty acids, eicosapentaenoic acid (20:5n-3) was found missing in the fish from polluted environment while it was found in considerable amount in farmed fish 7.8±0.4%. Docosahexaenoic acid (22:6n-3) also showed significant differences as 0.1±0.0 and 7.0±0.1% respectively, in wild polluted and farmed fishes.

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Authors and Affiliations

  1. Department of Zoology, Government College University, Faisalabad, Pakistan

    Bilal Hussain, Tayyaba Sultana, Salma Sultana & Shahid Mahboob

  2. Department of Zoology, College of Science, King Saud University, P. O. Box 2455, Riyadh, Saudi Arabia

    K. A. Al-Ghanim & Shahid Mahboob

Authors
  1. Bilal Hussain
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  2. Tayyaba Sultana
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  3. Salma Sultana
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  4. K. A. Al-Ghanim
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  5. Shahid Mahboob
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Corresponding author

Correspondence to Shahid Mahboob.

Additional information

Supported by the Research Group Project of Deanship of Scientific Research at King Saud University (No. RG-1435-012)

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Hussain, B., Sultana, T., Sultana, S. et al. Effect of pollution on DNA damage and essential fatty acid profile in Cirrhinus mrigala from River Chenab. Chin. J. Ocean. Limnol. 35, 572–579 (2017). https://doi.org/10.1007/s00343-017-5304-5

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  • DOI: https://doi.org/10.1007/s00343-017-5304-5

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